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four-parameter dose inhibition equation in graphpad prism 9.0  (GraphPad Software Inc)
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Four Parameter Dose Inhibition Equation In Graphpad Prism 9.0, supplied by GraphPad Software Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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4 parameter, nonlinear regression of dose response inhibition  (GraphPad Software Inc)
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GraphPad Software Inc 4 parameter, nonlinear regression of dose response inhibition
(A and B) The C M-H-L-5 blocked PRRSV Ch1a replication in Marc-145 cells. Marc-145 cells were infected with PRRSV Ch1a at an MOI of 0.1, and then treated with IFN-a (10 units/µl) or the C M-H-L-5 at various concentrations. At 24 h p.i., cells were fixed and analyzed by IFA using antibody against PRRSV N protein (A), and virus yield in the supernatants was also quantified (B). Culture treated with normal saline was set up as negative control (0 mg/ml), and treated with INF-α was set up as positive control. The brightness of the fluorescence represents the level of the virus. Results are from three independent experiments, each of which was conducted in triplicate. (C) The C M-H-L-5 potently inhibited both PRRSV HV and VR2332 replication in PAMs. A similar virus <t>inhibition</t> assay was performed with PAM cells infected with PRRSV strain HV or VR2332 at an MOI of 0.1 in the presence of IFN-a (10 units/µl) or the C M-H-L-5 at various concentrations. (D) Determination of cytotoxicity of the C M-H-L-5 by MTT assay. PAMs or Marc145 cells were incubated with various concentrations of the C M-H-L-5 or the control normal saline for 48 h prior to the MTT assay. Data are representative of three independent experiments (mean ± SD). Statistical significance was analyzed by Student’s t test. *P<0.05; **P<0.01; ***P<0.001.
4 Parameter, Nonlinear Regression Of Dose Response Inhibition, supplied by GraphPad Software Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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dose-response inhibition four-parameter variable slope equation  (GraphPad Software Inc)
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GraphPad Software Inc dose-response inhibition four-parameter variable slope equation
(A and B) The C M-H-L-5 blocked PRRSV Ch1a replication in Marc-145 cells. Marc-145 cells were infected with PRRSV Ch1a at an MOI of 0.1, and then treated with IFN-a (10 units/µl) or the C M-H-L-5 at various concentrations. At 24 h p.i., cells were fixed and analyzed by IFA using antibody against PRRSV N protein (A), and virus yield in the supernatants was also quantified (B). Culture treated with normal saline was set up as negative control (0 mg/ml), and treated with INF-α was set up as positive control. The brightness of the fluorescence represents the level of the virus. Results are from three independent experiments, each of which was conducted in triplicate. (C) The C M-H-L-5 potently inhibited both PRRSV HV and VR2332 replication in PAMs. A similar virus <t>inhibition</t> assay was performed with PAM cells infected with PRRSV strain HV or VR2332 at an MOI of 0.1 in the presence of IFN-a (10 units/µl) or the C M-H-L-5 at various concentrations. (D) Determination of cytotoxicity of the C M-H-L-5 by MTT assay. PAMs or Marc145 cells were incubated with various concentrations of the C M-H-L-5 or the control normal saline for 48 h prior to the MTT assay. Data are representative of three independent experiments (mean ± SD). Statistical significance was analyzed by Student’s t test. *P<0.05; **P<0.01; ***P<0.001.
Dose Response Inhibition Four Parameter Variable Slope Equation, supplied by GraphPad Software Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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nonlinear regression to a four parameter, variable slope, dose-response-inhibition function  (GraphPad Software Inc)
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GraphPad Software Inc nonlinear regression to a four parameter, variable slope, dose-response-inhibition function
(A and B) The C M-H-L-5 blocked PRRSV Ch1a replication in Marc-145 cells. Marc-145 cells were infected with PRRSV Ch1a at an MOI of 0.1, and then treated with IFN-a (10 units/µl) or the C M-H-L-5 at various concentrations. At 24 h p.i., cells were fixed and analyzed by IFA using antibody against PRRSV N protein (A), and virus yield in the supernatants was also quantified (B). Culture treated with normal saline was set up as negative control (0 mg/ml), and treated with INF-α was set up as positive control. The brightness of the fluorescence represents the level of the virus. Results are from three independent experiments, each of which was conducted in triplicate. (C) The C M-H-L-5 potently inhibited both PRRSV HV and VR2332 replication in PAMs. A similar virus <t>inhibition</t> assay was performed with PAM cells infected with PRRSV strain HV or VR2332 at an MOI of 0.1 in the presence of IFN-a (10 units/µl) or the C M-H-L-5 at various concentrations. (D) Determination of cytotoxicity of the C M-H-L-5 by MTT assay. PAMs or Marc145 cells were incubated with various concentrations of the C M-H-L-5 or the control normal saline for 48 h prior to the MTT assay. Data are representative of three independent experiments (mean ± SD). Statistical significance was analyzed by Student’s t test. *P<0.05; **P<0.01; ***P<0.001.
Nonlinear Regression To A Four Parameter, Variable Slope, Dose Response Inhibition Function, supplied by GraphPad Software Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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equation of four-parameter dose inhibition response  (GraphPad Software Inc)
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GraphPad Software Inc equation of four-parameter dose inhibition response
(A and B) The C M-H-L-5 blocked PRRSV Ch1a replication in Marc-145 cells. Marc-145 cells were infected with PRRSV Ch1a at an MOI of 0.1, and then treated with IFN-a (10 units/µl) or the C M-H-L-5 at various concentrations. At 24 h p.i., cells were fixed and analyzed by IFA using antibody against PRRSV N protein (A), and virus yield in the supernatants was also quantified (B). Culture treated with normal saline was set up as negative control (0 mg/ml), and treated with INF-α was set up as positive control. The brightness of the fluorescence represents the level of the virus. Results are from three independent experiments, each of which was conducted in triplicate. (C) The C M-H-L-5 potently inhibited both PRRSV HV and VR2332 replication in PAMs. A similar virus <t>inhibition</t> assay was performed with PAM cells infected with PRRSV strain HV or VR2332 at an MOI of 0.1 in the presence of IFN-a (10 units/µl) or the C M-H-L-5 at various concentrations. (D) Determination of cytotoxicity of the C M-H-L-5 by MTT assay. PAMs or Marc145 cells were incubated with various concentrations of the C M-H-L-5 or the control normal saline for 48 h prior to the MTT assay. Data are representative of three independent experiments (mean ± SD). Statistical significance was analyzed by Student’s t test. *P<0.05; **P<0.01; ***P<0.001.
Equation Of Four Parameter Dose Inhibition Response, supplied by GraphPad Software Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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prism 9.0 ‘dose-response-inhibition (four parameters  (GraphPad Software Inc)
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GraphPad Software Inc prism 9.0 ‘dose-response-inhibition (four parameters
(A and B) The C M-H-L-5 blocked PRRSV Ch1a replication in Marc-145 cells. Marc-145 cells were infected with PRRSV Ch1a at an MOI of 0.1, and then treated with IFN-a (10 units/µl) or the C M-H-L-5 at various concentrations. At 24 h p.i., cells were fixed and analyzed by IFA using antibody against PRRSV N protein (A), and virus yield in the supernatants was also quantified (B). Culture treated with normal saline was set up as negative control (0 mg/ml), and treated with INF-α was set up as positive control. The brightness of the fluorescence represents the level of the virus. Results are from three independent experiments, each of which was conducted in triplicate. (C) The C M-H-L-5 potently inhibited both PRRSV HV and VR2332 replication in PAMs. A similar virus <t>inhibition</t> assay was performed with PAM cells infected with PRRSV strain HV or VR2332 at an MOI of 0.1 in the presence of IFN-a (10 units/µl) or the C M-H-L-5 at various concentrations. (D) Determination of cytotoxicity of the C M-H-L-5 by MTT assay. PAMs or Marc145 cells were incubated with various concentrations of the C M-H-L-5 or the control normal saline for 48 h prior to the MTT assay. Data are representative of three independent experiments (mean ± SD). Statistical significance was analyzed by Student’s t test. *P<0.05; **P<0.01; ***P<0.001.
Prism 9.0 ‘Dose Response Inhibition (Four Parameters, supplied by GraphPad Software Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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prism 9.0 ‘dose-response-inhibition (four parameters - by Bioz Stars, 2026-03
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(A and B) The C M-H-L-5 blocked PRRSV Ch1a replication in Marc-145 cells. Marc-145 cells were infected with PRRSV Ch1a at an MOI of 0.1, and then treated with IFN-a (10 units/µl) or the C M-H-L-5 at various concentrations. At 24 h p.i., cells were fixed and analyzed by IFA using antibody against PRRSV N protein (A), and virus yield in the supernatants was also quantified (B). Culture treated with normal saline was set up as negative control (0 mg/ml), and treated with INF-α was set up as positive control. The brightness of the fluorescence represents the level of the virus. Results are from three independent experiments, each of which was conducted in triplicate. (C) The C M-H-L-5 potently inhibited both PRRSV HV and VR2332 replication in PAMs. A similar virus inhibition assay was performed with PAM cells infected with PRRSV strain HV or VR2332 at an MOI of 0.1 in the presence of IFN-a (10 units/µl) or the C M-H-L-5 at various concentrations. (D) Determination of cytotoxicity of the C M-H-L-5 by MTT assay. PAMs or Marc145 cells were incubated with various concentrations of the C M-H-L-5 or the control normal saline for 48 h prior to the MTT assay. Data are representative of three independent experiments (mean ± SD). Statistical significance was analyzed by Student’s t test. *P<0.05; **P<0.01; ***P<0.001.

Journal: PLoS ONE

Article Title: A Novel Compound from the Mushroom Cryptoporus volvatus Inhibits Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) In Vitro

doi: 10.1371/journal.pone.0079333

Figure Lengend Snippet: (A and B) The C M-H-L-5 blocked PRRSV Ch1a replication in Marc-145 cells. Marc-145 cells were infected with PRRSV Ch1a at an MOI of 0.1, and then treated with IFN-a (10 units/µl) or the C M-H-L-5 at various concentrations. At 24 h p.i., cells were fixed and analyzed by IFA using antibody against PRRSV N protein (A), and virus yield in the supernatants was also quantified (B). Culture treated with normal saline was set up as negative control (0 mg/ml), and treated with INF-α was set up as positive control. The brightness of the fluorescence represents the level of the virus. Results are from three independent experiments, each of which was conducted in triplicate. (C) The C M-H-L-5 potently inhibited both PRRSV HV and VR2332 replication in PAMs. A similar virus inhibition assay was performed with PAM cells infected with PRRSV strain HV or VR2332 at an MOI of 0.1 in the presence of IFN-a (10 units/µl) or the C M-H-L-5 at various concentrations. (D) Determination of cytotoxicity of the C M-H-L-5 by MTT assay. PAMs or Marc145 cells were incubated with various concentrations of the C M-H-L-5 or the control normal saline for 48 h prior to the MTT assay. Data are representative of three independent experiments (mean ± SD). Statistical significance was analyzed by Student’s t test. *P<0.05; **P<0.01; ***P<0.001.

Article Snippet: The 50% effective concentration (EC 50 ) was determined using a 4 parameter, nonlinear regression of dose response inhibition by plotting log (inhibitor(-concentration)) vs. virus titer (variable slope) using GraphPad Prism (GraphPad Software, San Diego, CA).

Techniques: Infection, Virus, Saline, Negative Control, Positive Control, Fluorescence, Inhibition, MTT Assay, Incubation, Control